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KMID : 0363320060270010178
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Journal of Korean Oriental Internal Medicine
2006 Volume.27 No. 1 p.178 ~ p.187
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Peroxynitrite Scavenging Activity of Samjunghwan
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Lee Dong-Joon
Jung Ji-Chun
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Abstract
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Objectives : Peroxynitrite (ONOO -),;{cdot}O_2- and nitric oxide (NO) are cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer’s disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate ONOO- scavengactivities, and that of its precursors, NO and {cdot}O_2- of Samjunghwan.
Methods : To investigate ONOO-,;NO,;{cdot}O_2- scavenging activities, fluorescent probes, namely 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used.
Results : Samjunghwan markedly scavenged authentic ONOO-,;{cdot}O_2- and NO. It also inhibited ONOO- induced by .02’ and NO which are derived from SIN-I. Furthermore, Samjunghwan inhibited ONOO-, {cdot}O_2- and NO generation in LPS-treated ICR mouse kidney postmitochondria.
Conclusions : These results suggest that Samjunghwan is an effective ONOO-,;{cdot}O_2- and NO scavenger, and that this substance has a potential role as an inhibitor of aging process, and in therapy against age-related diseases.
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KEYWORD
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Samjunghwan, peroxynitrite, nitric oxide, reactive oxygen species
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